6533b830fe1ef96bd1297164

RESEARCH PRODUCT

Transformation of Aspergillus parasiticus using autonomously replicating plasmids from Aspergillus nidulans.

C.j. BosAlfons J. M. DebetsCristina PascualMiguel A. MorenoAlicia GibelloGuillermo SuárezSergi Ferrer

subject

DNA ReplicationArginine BAutonomously replicating sequenceMitosisLaboratorium voor ErfelijkheidsleerMicrobiologyAspergillus parasiticusAspergillus nidulansMicrobiologyGenetic transformationchemistry.chemical_compoundPlasmidTransformation GeneticAutonomous replicationAspergillus nidulansGeneticsDNA FungalMolecular BiologyGeneGeneticsAspergillus nidulans autonomous replicating plasmidbiologyProtoplastsfood and beveragesProtoplastbiology.organism_classificationAspergillus parasiticusTransformation (genetics)AspergilluschemistryLaboratory of GeneticsDNAPlasmids

description

A genetic transformation system for the aflatoxin-producing fungus Aspergillus parasiticus using two autonomously replicating plasmids from A. nidulans (ARp1 and pDHG25) is reported. Transformation frequencies using the plasmid pDHG25 were from 5 x 10(2) to 2.5 x 10(4) transformants per 10(6) viable protoplasts and microgram DNA. The stability of the plasmids in the transformants was also studied. This transformation system offers a new opportunity to clone genes related to aflatoxin production using appropriate aflatoxin-defective mutants.

yearjournalcountryeditionlanguage
1994-01-01FEMS microbiology letters
10.1111/j.1574-6968.1994.tb07258.xhttps://pubmed.ncbi.nlm.nih.gov/8001767