6533b830fe1ef96bd129719b
RESEARCH PRODUCT
Laser speckle imaging for early detection of microbial colony forming units
Janis LiepinsIlze LihacovaIlya BalmagesAlexey LihachevStivens ZolinsDmitrijs Bliznukssubject
Colony-forming unit0303 health sciencesSignal processingMaterials sciencebiologybusiness.industryGompertz functionEarly detectionLaser Speckle ImagingVibrio natriegensbiology.organism_classification01 natural sciencesArticleAtomic and Molecular Physics and Optics010309 optics03 medical and health sciencesSpeckle patternOptics0103 physical sciencesCorrelation analysisbusiness030304 developmental biologyBiotechnologydescription
In this study, an optical contactless laser speckle imaging technique for the early identification of bacterial colony-forming units was tested. The aim of this work is to compare the laser speckle imaging method for the early assessment of microbial activity with standard visual inspection under white light illumination. In presented research, the growth of Vibrio natriegens bacterial colonies on the solid medium was observed and analyzed. Both – visual examination under white light illumination and laser speckle correlation analysis were performed. Based on various experiments and comparisons with the theoretical Gompertz model, colony radius growth curves were obtained. It was shown that the Gompertz model can be used to describe both types of analysis. A comparison of the two methods shows that laser speckle contrast imaging, combined with signal processing, can detect colony growth earlier than standard CFU counting method under white light illumination.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-02-24 | Biomedical Optics Express |