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RESEARCH PRODUCT
Triacylglycerol Analysis in Human Milk and Other Mammalian Species: Small-Scale Sample Preparation, Characterization, and Statistical Classification Using HPLC-ELSD Profiles.
Eduardo Beltrán-ituratErnesto F. Simó-alfonsoIsabel Ten-doménechJosé Manuel Herrero-martínezJuan Vicente Sancho-llopissubject
mammalian milk triacylglycerolslinear discriminant analysisResolution (mass spectrometry)HPLC-ELSDBiologyHigh-performance liquid chromatographyMass SpectrometryChromatography detectorAnimalsHumansSample preparationChromatography High Pressure LiquidTriglyceridesfat extractionPacked bedChromatography Reverse-PhaseChromatographySheepMilk HumanGoatsExtraction (chemistry)human milkDiscriminant AnalysisGeneral ChemistryStatistical classificationMilkComposition (visual arts)CattleFemaleGeneral Agricultural and Biological Sciencesdescription
In this work, a method for the separation of triacylglycerols (TAGs) present in human milk and from other mammalian species by reversed-phase high-performance liquid chromatography using a core–shell particle packed column with UV and evaporative light-scattering detectors is described. Under optimal conditions, a mobile phase containing acetonitrile/n-pentanol at 10 °C gave an excellent resolution among more than 50 TAG peaks. A small-scale method for fat extraction in these milks (particularly of interest for human milk samples) using minimal amounts of sample and reagents was also developed. The proposed extraction protocol and the traditional method were compared, giving similar results, with respect to the total fat and relative TAG contents. Finally, a statistical study based on linear discriminant analysis on the TAG composition of different types of milks (human, cow, sheep, and goat) was carried out to differentiate the samples according to their mammalian origin.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-06-10 | Journal of agricultural and food chemistry |