6533b830fe1ef96bd1297916
RESEARCH PRODUCT
Determination of clenbuterol in urine by azo-dye precolumn derivatization and micellar liquid chromatography
J. J. Baeza BaezaGuillermo Ramis-ramosY.martin Bioscasubject
Detection limitChromatographyChemistryElutionOrganic ChemistryClinical BiochemistryExtraction (chemistry)Reversed-phase chromatographyBiochemistryHigh-performance liquid chromatographyAnalytical Chemistrychemistry.chemical_compoundMicellar liquid chromatographySolid phase extractionDerivatizationdescription
Clenbuterol has been determined in urine by solidphase extraction on a C18 cartridge, diazotization of the eluate with nitrite, coupling of the diazonium ion with 1-(naphthyl)ethylenediamine, and separation of the azo dye formed by HPLC with a C18 column and a micellar mobile phase containing 0.1 M sodium dodecyl sulphate, 12%n-butanol and 0.05 M citrate buffer, pH 3. Recoveries higher than 90% were obtained by mixing the samples with a 20% 0.2 M NaOH before extraction. Limits of detection of 51 and 6.7 ng L−1 were obtained with spectrophotometric and thermal lens spectrometric detection, respectively; respective repeatabilities were 3.1% (5 μg mL−1) and 5.6% (0.16 μg mL−1).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-02-01 | Chromatographia |