6533b830fe1ef96bd1297962

RESEARCH PRODUCT

Effect of α-factor on individual wall mannoproteins fromSaccharomyces cerevisiae acells

Eulogio ValentínEnrique HerreroRafael Sentandreu

subject

Gel electrophoresisbiologySaccharomyces cerevisiaebiology.organism_classificationMicrobiologyYeastcarbohydrates (lipids)Cell wallAgglutination (biology)Endoglycosidase Hchemistry.chemical_compoundBiochemistrychemistryMannosylationAcrylamideGeneticsbiology.proteinMolecular Biology

description

Treatment of Saccharomyces cerevisiae a cells with α-factor partially inhibits mannosylation of the high Mr mannoproteins, although there is an increase in the total amount of these molecules present in the wall. They show a similar mobility in SDS-acrylamide gels to those from untreated mnn2 cells. No other significant effects on wall mannoproteins have been observed, except a decrease in the amount of the 29 kDa species.

https://doi.org/10.1111/j.1574-6968.1985.tb00685.x