A rapid procedure for the determination of caffeine, theophylline and theobromine in urine by micellar liquid chromatography and direct sample injection

6533b830fe1ef96bd1297998

RESEARCH PRODUCT

A rapid procedure for the determination of caffeine, theophylline and theobromine in urine by micellar liquid chromatography and direct sample injection

Salvador Sagrado I. Perez-martinez M.j. Medina-hernández

subject

Detection limit Chromatography Chemistry Urine Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Column chromatography Micellar liquid chromatography medicine Environmental Chemistry Theophylline Caffeine Theobromine Spectroscopy medicine.drug

description

Abstract A liquid chromatographic procedure for the determination of caffeine, theophylline and theobromine in urine samples is described. The proposed system uses a Spherisorb octadecyl-silane ODS-2 C18 analytical column and a guard column of similar characteristics. The UV detector was set at 273 nm. A study to adequately select the composition of the micellar mobile phase for the separation of these compounds in urine samples is performed. Maximum resolution was achieved with a 0.075 M sodium dodecylsulphate-1.5% propanol eluent. Limits of detection at 273 nm ranged between 0.4 μg/ml for theobromine and theophylline and 1.2 μg/ml for caffeine. The procedure allows the determination of these compounds in 10 min and it can be very useful in the control of doping.

year	journal	country	edition	language
1995-03-01	Analytica Chimica Acta

https://doi.org/10.1016/0003-2670(94)00619-w

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