6533b830fe1ef96bd12979ae
RESEARCH PRODUCT
Towards the optimization of complementary systems in reversed-phase liquid chromatography
M.c. García-alvarez-coqueJose Ramon Torres-lapasioG. Vivó-truyolssubject
ChromatographyChemistrybusiness.industryOrganic solventPhase compositionOrganic ChemistryClinical BiochemistryReversed-phase chromatographyProcess engineeringbusinessBiochemistryHigh-performance liquid chromatographyAnalytical Chemistrydescription
Previously reported optimization methodology, which seeks complementary mobile phases (CMP) in isocratic chromatography, has been extended to include more than one system simultaneously (i.e. more than one organic solvent and/or column). In the literature the benefits of complementarity are not usually fully exploited—few working conditions giving rise to interactions as different as possible are examined, without developing a fully linked optimization. The proposed approach is compared critically with use of a single mobile phase or CMP which consider one system only. The strategy greatly expands the capability of isocratic chromatography in the analysis of complex samples that cannot be resolved by use of conventional methods.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-12-01 | Chromatographia |