6533b831fe1ef96bd12984c9
RESEARCH PRODUCT
Somatic embryogenesis and plant regeneration in a woody species: the European Spindle Tree (Euonymus europaeus L.).
Laurent BonneauN. Béranger-novatJ. Moninsubject
chemistry.chemical_classificationanimal structuresSomatic embryogenesisfungiEmbryogenesisfood and beveragesEmbryoPlant ScienceGeneral MedicineBiologybiology.organism_classificationchemistry.chemical_compoundTissue culturechemistryAuxinCallusembryonic structuresBotanyEuonymus europaeusKinetinAgronomy and Crop Sciencedescription
Somatic embryogenesis and subsequent plant regeneration of Euonymus europaeus L (European Spindle Tree) were obtained from square pieces of mature zygotic embryos with an intervening callus phase. Callus and somatic embryos were induced using a Murashige and Skoog's semi-solid basal medium supplemented with several combinations of auxins and cytokinins. The greatest number of somatic embryos was obtained with a continuous exposure to 22.8 μM indoleacetic acid and 0.046 μM kinetin. The frequency of somatic embryogenesis from zygotic embryos depends on the cold conservation time of seeds. The embryos frequently germinated on the same medium. Further development of somatic embryos into plantlets was achieved on a medium devoid of growth regulators.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-01-06 | Plant cell reports |