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RESEARCH PRODUCT
The mitochondrial genome of fission yeast: inability of all introns to splice autocatalytically, and construction and characterization of an intronless genome
Bernd SchäferCarola AnderlKlaus Werner WolfFranz WelserManfred ZimmerAna Maria Merlos-langesubject
Mitochondrial DNARNA MitochondrialRNA SplicingMolecular Sequence DataBiologyDNA MitochondrialGenomeElectron Transport Complex IVConsensus SequenceSchizosaccharomycesGeneticsGroup I catalytic intronAmino Acid SequenceCloning MolecularMolecular BiologyGeneGeneticsBase SequenceIntronRNAGroup II intronCytochromes bCytochrome b GroupIntronsMitochondriaRNA splicingNucleic Acid ConformationRNAApoproteinsdescription
In this paper we report the inability of four group I introns in the gene encoding subunit I of cytochrome c oxidase (cox1) and the group II intron in the apocytochrome b gene (cob) to splice autocatalytically. Furthermore we present the characterization of the first cox1 intron in the mutator strain anar-14 and the construction and characterization of strains with intronless mitochondrial genomes. We provide evidence that removal of introns at the DNA level (termed DNA splicing) is dependent on an active RNA maturase. Finally we demonstrate that the absence of introns does not abolish homologous mitochondrial recombination.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-01-01 | Molecular and General Genetics MGG |