6533b831fe1ef96bd129874f

RESEARCH PRODUCT

Studies on the mechanism of PMN activation II. by triggering the alternative pathway of complement activation

J. MüllerU. BotzenhardtE. M. Lemmel

subject

medicine.medical_specialtyNeutrophilsPhagocytosisComplement Pathway AlternativeDose-Response Relationship ImmunologicStimulationMonocyteschemistry.chemical_compoundPhagocytosisInternal medicinemedicineHumansComplement ActivationHematologyChemistryDextran SulfateZymosanZymosanDextransComplement C3HematologyGeneral MedicinePeptide FragmentsCell biologyComplement systemDextran sulfateBiochemistryComplement C3aAlternative complement pathwayCobra venom factor

description

By means of cobra venom factor (CVF) it is demonstrated that the stimulation of hexosemonophosphate shunt (HMPS) of human polymorphonuclear leukocytes (PMN) by zymosan (Z) and dextran sulfate (DS) is caused by at least two modes of activation: (a) via activation caused by phagocytosis, (b) via activated alternative pathway of complement activation (APC). Active factors of APC presented with phagocytizable objects strongly enhance activation of PMN. The effect of APC can be observed in serum-containing as well as in serum-free cultures. It can be demonstrated that in serum-free cultures the factors of APC participating in the activation of PMN are supplied by monocytes. By the use of synthetic hexapeptide (HP) representing the COOH-terminal sequence of human C3a further evidence is provided that factors of APC are able to activate the PMN.

yearjournalcountryeditionlanguage
1982-07-01Blut
https://doi.org/10.1007/bf00320494