6533b831fe1ef96bd1298f4c

RESEARCH PRODUCT

Dispersive magnetic immunoaffinity extraction. Anatoxin-a determination.

Sergio ArmentaTinh LeFrancesc A. Esteve-turrillasGuillermo Quiñones-reyesMiguel De La GuardiaAntonio Abad-somovillaAntonio Abad-fuentes

subject

Ion-mobility spectrometryRelative standard deviationAnalytical chemistry02 engineering and technology01 natural sciencesBiochemistryAnalytical ChemistryAnatoxin-aSepharosechemistry.chemical_compoundMagneticsLimit of DetectionIon Mobility SpectrometryDetection limitChromatographyCyanobacteria ToxinsSepharose010401 analytical chemistryOrganic ChemistryExtraction (chemistry)Antibodies MonoclonalWaterGeneral Medicine021001 nanoscience & nanotechnology0104 chemical scienceschemistryIsobaric process0210 nano-technologyWater Pollutants ChemicalEnvironmental MonitoringTropanes

description

Specific monoclonal antibodies were coupled with magnetic Sepharose-based beads and used, for the first time. The methodology was applied to preconcentrate anatoxin-a from water and the later determination by ion mobility spectrometry (IMS). Dispersive magnetic immunoaffinity (d-MagIA) extraction methodology provided a limit of detection of 0.02μgL-1 and a satisfactory precision with a relative standard deviation lower than 15%. Recoveries were evaluated at 0.5, 1.0 and 5.0μgL-1 anatoxin-a with quantitative values from 91 to 115%. Additionally, isobaric interferences with phenylalanine were completely avoided by the use of the developed d-MagIA extraction coupled to IMS determinations.

yearjournalcountryeditionlanguage
2017-09-18Journal of chromatography. A
10.1016/j.chroma.2017.10.076https://pubmed.ncbi.nlm.nih.gov/29126587