6533b831fe1ef96bd12996a1

RESEARCH PRODUCT

Factors Influencing Digitalis Obscura Mesophyll Protoplast Development

J. SeguraM. C. BrisaR. Jover

subject

Casein hydrolysatechemistry.chemical_compoundbiologychemistryBotanyDigitalis obscuraAgaroseProtoplastbiology.organism_classificationSolid medium

description

In a previous work (1), we found that a combination of liquid-over-agar-solidified MS (2) modified medium was superior to either all liquid or all solid medium for culture of Digitalis obscura L. mesophyll protoplasts. In order to achieve optimum culture conditions we now report on the influence of a different gelling agent (agarose) as well as the effect of several nutrient media on D. obscura mesophyll protoplasts development.

https://doi.org/10.1007/978-94-009-2788-9_39