6533b831fe1ef96bd1299721

RESEARCH PRODUCT

Somatic Embryogenesis and Plant Regeneration from Hypocotyl Cultures of Digitalis obscura L.

M. C. BrisaJuan SeguraIsabel Arrillaga

subject

photoperiodismbiologySomatic embryogenesisPhysiologyEmbryogenesisDigitalis obscurafood and beveragesEmbryoPlant Sciencebiology.organism_classificationHypocotylTissue cultureBotanyAgronomy and Crop ScienceIncubation

description

Summary Hypocotyl sections from 20-day-old seedlings of Digitalis obscura L. were used to examine the effect of IAA on somatic embryogenesis. Although embryogenesis was obtained with all IAA concentrations (0.1, 0.5 and 1 ppm) and under both illumination conditions tested (16 h photoperiod and darkness), incubation in photoperiod increased yield of embryos and shortened the embryo induction time. Optimum results were obtained with 1 ppm IAA. About 30 % of the embryos obtained developed into complete plantlets when transferred to basal medium without growth regulators.

yearjournalcountryeditionlanguage
1986-07-01Journal of Plant Physiology
https://doi.org/10.1016/s0176-1617(86)80200-0