6533b832fe1ef96bd129a592
RESEARCH PRODUCT
Fishing for G-quadruplexes in solution with a perylene diimide derivative labeled with biotins
Giampaolo BaroneJavier SantolayaJavier SantolayaJosé M. LealAurore GuédinBegoña GarcíaNatalia BustoTomás TorrobaJean-louis MergnyPatricia Calvosubject
0301 basic medicinePerilipin-1Surface PropertiesBiotinDNA Single-StrandedImidesLigandsCatalysisCatalysi03 medical and health sciencesheterocyclic compoundsPeryleneG-quadruplexeFluorescent DyesPerileneChemistryOrganic ChemistryQuímicaGeneral ChemistryDNAG-QuadruplexesSolutionsChemistry030104 developmental biologyBiophysicNucleic acidMagnetsChristian ministryStreptavidinHumanitiesdescription
A new fluorescent, non‐cytotoxic perylene diimide derivative with two biotins at the peri position, PDI2B, has been synthesized. This molecule is able to interact selectively with G‐quadruplexes with scarce or no affinity towards single‐ or double‐stranded DNA. These features have made it possible to design a simple, effective, safe, cheap, and selective method for fishing G‐quadruplex structures in solution by use of PDI2B and streptavidin coated magnetic beads. The new cyclic method reported leads to the recovery of more than 80 % of G‐quadruplex structures from solution, even in the presence of an excess of single‐stranded or duplex DNA as competitors. Moreover, PDI2B is a G4 ligand that can display higher thermal stabilization and greater affinity for 2‐ over 3‐tetrad quadruplexes, which constitutes a novel type of behavior.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-01-01 |