6533b833fe1ef96bd129b881

RESEARCH PRODUCT

Effect of sulfur dioxide on mucociliary activity and ciliary beat frequency in guinea pig trachea

Kienast KMarli Maria KnorstHerbert RiechelmannFerlinz RJ. Müller-quernheim

subject

Baseline valuesDose-Response Relationship DrugInhalationMucociliary clearanceCiliary activityGuinea PigsPublic Health Environmental and Occupational Healthcomplex mixturesTracheaAndrologyGuinea pigchemistry.chemical_compoundchemistryMucociliary ClearanceAnesthesiaToxicitycardiovascular systemAnimalsSulfur DioxideCiliary beatingSulfur dioxideCiliary Motility Disorderscirculatory and respiratory physiology

description

The effects of 30 min exposure to sulfur dioxide on mucociliary activity (MCA) and ciliary beat frequency (CBF) were studied in 31 guinea pig tracheas. MCA was measured by recording the light reflected from ciliated mucous membranes using an infrared bar code reader. CBF of single ciliated cells obtained by brushing was measured with phase-contrast microscopy. Each tracheal sample was exposed to SO2 at concentrations ranging from 2.5 to 12.5 ppm, or to air for control purposes. MCA and CBF were measured before and immediately after gas exposure. A reduction in mean MCA of 63% (P = 0.0007) and statistically insignificant changes in CBF (P > 0.05) were recorded at concentrations of 2.5 PPM SO2. Higher SO2 concentrations caused a further impairment of MCA as well as a dose-dependent decrease in CBF (P = 0.002). A concentration of 12.5 PPM SO2 induced a decrease from baseline values of approximately 80% in mean MCA and of roughly 70% in mean CBE This study demonstrates a dose-dependent SO2-induced decrease in MCA of guinea pig tracheas. The decrease in MCA was associated with an impairment of CBF only at SO2 concentrations higher than 5.0 ppm.

yearjournalcountryeditionlanguage
1994-02-01International Archives of Occupational and Environmental Health
https://doi.org/10.1007/bf00405697