6533b833fe1ef96bd129ba18

RESEARCH PRODUCT

Molecular Characterization of Fusarium oxysporum f. melongenae by ISSR and RAPD Markers on Eggplant

Francesco CarimiS. ZenginMirko SiragusaÖMür BaysalMaurizio SajevaEmine GümrükcüJaime A. Teixeira Da Silva

subject

Genetic MarkersFusariumVeterinary medicineSettore BIO/11 - Biologia MolecolareMinisatellite RepeatsMolecular markerBiochemistryFusarium molecular marker virulence genetic differencechemistry.chemical_compoundFusariumMolecular markerBotanyGenetic variationFusarium oxysporumGeneticsSolanum melongenaDNA FungalMolecular BiologyEcology Evolution Behavior and SystematicsDNA PrimersVirulencebiologyGenetic Variationfood and beveragesGeneral Medicinebiology.organism_classificationRandom Amplified Polymorphic DNA TechniqueRAPDGenetic differencechemistryGenetic markerSettore BIO/03 - Botanica Ambientale E ApplicataMicrosatelliteSolanum

description

Fusarium oxysporum f. melongenae is a major soil-borne pathogen of eggplant (Solanum melongena). ISSR and RAPD markers were used to characterize Fusarium oxysporum f. melongenae isolates collected from eggplant fields in southern Turkey. Those isolates were not pathogenic to tomato. Pathogens were identified by their morphology, and their identity was confirmed by PCR amplifi- cation using the specific primer PF02-3. The isolates were classified into groups on the basis of ISSR and RAPD fingerprints, which showed a level of genetic speci- ficity and diversity not previously identified in Fusarium oxysporum f. melongenae, suggesting that genetic differences are related to the pathogen in the Mediterranean region. The primers selected to characterize Fusarium oxysporum f. melongenae may be used to determine genetic differences and pathogen virulence. This study is the first to characterize eggplant F. oxysporum species using ISSR and RAPD.

yearjournalcountryeditionlanguage
2010-01-01Biochemical Genetics
https://doi.org/10.1007/s10528-010-9336-1