Žurku smadzeņu stumbra un garozas rajonu astrocītu funkcionālā heterogenitāte

6533b833fe1ef96bd129bc71

RESEARCH PRODUCT

Žurku smadzeņu stumbra un garozas rajonu astrocītu funkcionālā heterogenitāte

Vladimirs Piļipenko

subject

description

Šī darba mērķis bija noteikt žurku smadzeņu stumbra un garozas astrocītu funkcionālo heterogenitāti, balstoties uz Na+/HCO3- kotransporteru NBCe1, NBCn1, NBCe2 un šķīstošās adenililciklāzes (sAC) gēnu ekspresijas profiliem. Iegūtie rezultāti parādīja, ka individuālos astrocītos no smadzeņu stumbra, salīdzinot ar ekspresiju smadzeņu garozas astrocītos a) sAC gēnu ekspresija bija 1,35 reizes zemāka (p<0,01); b) NBCe1 un NBCn1 gēnu ekspresijai bija tendence būt lielākai (R=2,44 un 1,18, attiecīgi) un c) NBCe2 gēnu ekspresija bija 4 reizes augstāka (p<0,05). Šie dati liecina, ka pastāv funkcionāla heterogenitāte starp smadzeņu stumbra un garozas astrocītiem. Smadzeņu stumbra astrocītu specifiskais jutīgums pret pH varētu būt attiecināms uz mehānismiem, kas nav mediēti caur NBCe1 atkarīgu HCO3− ieplūdi astrocītu šūnās un sAC aktivēšanos. Atslēgvārdi: astrocīti, NBC, pH homeostāze, qPCR-RT, sAC.

year	journal	country	edition	language
2014-01-01

https://dspace.lu.lv/dspace/handle/7/20429

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