6533b833fe1ef96bd129c356

RESEARCH PRODUCT

Quantitative determination of casein genetic variants in goat milk: Application in Girgentana dairy goat breed

Maria MontalbanoRosalia Di GerlandoSalvatore MastrangeloRoberta SegretoMaria Teresa Sardina

subject

animal structuresGenotypeGenetic variants; caseins; HPLC; Goat milk.Biology01 natural sciencesHigh-performance liquid chromatographycaseinAnalytical ChemistrySettore AGR/17 - Zootecnica Generale E Miglioramento GeneticoCaseinGenotypeGenetic variationAnimalsFood scienceAlleleGenetic variantAllelesChromatography High Pressure LiquidGoat milk.ChromatographyGoats010401 analytical chemistry0402 animal and dairy scienceGenetic variantsCaseinsGenetic VariationReproducibility of Results04 agricultural and veterinary sciencesGeneral Medicine040201 dairy & animal scienceBreedQuantitative determination0104 chemical sciencesMilkEvaluation Studies as TopicHPLCFood Science

description

The study was conducted to develop a high-performance liquid chromatographic (HPLC) method to quantify casein genetic variants (αs2-, β-, and κ-casein) in milk of homozygous individuals of Girgentana goat breed. For calibration experiments, pure genetic variants were extracted from individual milk samples of animals with known genotypes. The described HPLC approach was precise, accurate and highly suitable for quantification of goat casein genetic variants of homozygous individuals. The amount of each casein per allele was: αs2-casein A = 2.9 ± 0.8 g/L and F = 1.8 ± 0.4 g/L; β-casein C = 3.0 ± 0.8 g/L and C1 = 2.0 ± 0.7 g/L and κ-casein A = 1.6 ± 0.3 g/L and B = 1.1 ± 0.2 g/L. A good correlation was found between the quantities of αs2-casein genetic variants A and F, and β-casein C and C1 with other previously described method. The main important result was obtained for κ-casein because, till now, no data were available on quantification of single genetic variants for this protein.

yearjournalcountryeditionlanguage
2016-02-01
http://hdl.handle.net/10447/144612