Sigma-1 receptora gēna ietekme uz peļu taukaudu šūnu diferenciāciju par adipocītiem

6533b833fe1ef96bd129c5e7

RESEARCH PRODUCT

Sigma-1 receptora gēna ietekme uz peļu taukaudu šūnu diferenciāciju par adipocītiem

Viktorija Silantjeva

subject

adipoģenēze Sigma-1 receptors adipocīti Farmācija mezenhimālās stromas šūnas

description

No taukiem iegūtas mezenhimālās stromas šūnas (AdMSŠ) spēj diferencēties par adipocītiem. Pateicoties diferenciācijas potenciālam, tām ir plašs terapeitisko pielietojumu loks reģeneratīvajā medicīnā. Bakalaura darba mērķis bija izpētīt Sigma-1 (S1R) receptora gēna ietekmi uz peļu taukaudu šūnu diferenciāciju par adipocītiem in vitro. Lai analizētu adipodiferenciāciju AdMSŠ un S1R nozīmi, tika pārbaudīts četru ar taukaudiem saistītu gēnu CEBPA, PPARG, FABP4 un Adiponectin ekspresijas līmenis, kas tika analizēts ar qPCR. MSŠ diferencējas par nobriedušām adipocītu šūnām, ko apstiprina adipodiferenciācijai raksturīgo marķieru FABP4 un Adiponectin ekspresijas paaugstināšanās. Rezultāti norāda, ka nav būtiskas atšķirības MSŠ marķieru ekspresijas profilā starp WT un S1R-/- pēc adipodifereciācijas. Tendence uz samazinātu ar adipocītiem saistītu marķieru ekspresiju S1R-/-, salīdzinot ar WT nediferencētā un diferencētā stāvoklī, norāda, ka S1R varētu būt iesaistīts adipoģenēzes regulēšanā.

year	journal	country	edition	language
2021-01-01

https://dspace.lu.lv/dspace/handle/7/54706

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