6533b834fe1ef96bd129cbe5
RESEARCH PRODUCT
Azo-Sulforhodamine Dyes: A Novel Class of Broad Spectrum Dark Quenchers
Anthony RomieuAnthony RomieuPierre-yves RenardArnaud Chevaliersubject
Spectrophotometry InfraredPhotochemistryBiochemistryBroad spectrumchemistry.chemical_compoundAnilineRapid accessDark quencher[CHIM]Chemical SciencesPhysical and Theoretical ChemistryColoring AgentsComputingMilieux_MISCELLANEOUSFluorescent DyesAniline CompoundsQuenching (fluorescence)Molecular StructureRhodaminesOrganic ChemistryWaterSulforhodamine 101Fluorescence3. Good healthFörster resonance energy transferSolubilitychemistryAzo Compoundsdescription
A rapid access to a novel class of water-soluble dark quencher dyes was achieved using an azo-coupling reaction between a fluorescent primary arylamine derived from a sulforhodamine 101 scaffold and a tertiary aniline equipped with different bioconjugatable groups. The thus obtained nonfluorescent azo-sulforhodamine hybrids display a broad quenching range spanning the visible to NIR regions. This was demonstrated through the preparation and enzymatic activation of FRET-based fluorogenic substrates of urokinase.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-07-02 |