6533b834fe1ef96bd129cc8a
RESEARCH PRODUCT
A rapid and simple multiparameter assay to quantify spike-specific CD4 and CD8 T cells after SARS-CoV-2 vaccination: A preliminary report
Francesco DieliAntonino TrizzinoNadia CaccamoGiusto Davide BadamiMarco Pio La MannaPaolo RagoneseMojtaba Shekarkar Azgomisubject
CD4<sup>+</sup>CD8<sup>+</sup>QH301-705.5medicine.medical_treatmentT cellT cellsMedicine (miscellaneous)Cytotoxic cellsMemory T cellGeneral Biochemistry Genetics and Molecular BiologyCD4+Immune systemImmunityCytotoxic T cellMedicineBiology (General)B cellSettore MED/04 - Patologia Generalebusiness.industrySARS-CoV-2CommunicationCD8CD8+CD4Pfizer/BioNTechmedicine.anatomical_structureCytokineImmunologyCytokinesbusinessMemory T cellCD8description
mRNA and Adenovirus vaccines for COVID-19 are used to induce humoral and cell-mediated immunity, with the aim to generate both SARS-CoV-2 B and T memory cells. In present study, we described a simple assay to detect and quantify Spike-specific CD4+ and CD8+ T cell responses induced by vaccination in healthy donors and in subjects with B cell compart impairment, in which antibody response is absent due to primary immunodeficiencies or CD20 depleting therapy. We detect and quantified memory T cell immune responses against SARS-CoV-2 evocated by vaccination in both groups, irrespective to the humoral response. Furthermore, we identified TNF-α as the main cytokine produced by T memory cells, after antigen-specific stimulation in vitro, that could be considered, other than IFN-γ, an additional biomarker of induction of T memory cells upon vaccination. Further studies on the vaccine-induced T cell responses could be crucial, not only in healthy people but also in immunocompromised subjects, where antigen specific T cells responses play a protective role against SARS-CoV-2.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-10-01 |