6533b834fe1ef96bd129cddb
RESEARCH PRODUCT
Proteomics as a tool to monitor plant-microbe endosymbioses in the rhizosphere
Silvio GianinazziEliane Dumas-gaudotGwénaëlle Bestel-corresubject
Proteomics0106 biological sciencesPlant ScienceComputational biologyBiologyProteomicsPlant Roots01 natural sciencesMass SpectrometryFungal Proteins03 medical and health sciencesTwo dimensional electrophoresisMycorrhizaeBotanyGeneticsSymbiosisMolecular BiologySoil MicrobiologyEcology Evolution Behavior and Systematics[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/MycologyComputingMilieux_MISCELLANEOUSPlant Proteins030304 developmental biology0303 health sciencesRhizospherePlant microbeGeneral Medicine[SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/MycologyInvestigation methods010606 plant biology & botanydescription
In recent years, outstanding molecular approaches have been used to investigate genes and functions involved in plant-microbe endosymbioses. In this review, we outline the use of proteomic analysis, based on two-dimensional electrophoresis and mass spectrometry, to characterize symbiosis-related proteins. During the last decade, proteomics succeeded in identifying about 400 proteins associated with the development and functioning of both mycorrhizal and rhizobial symbioses. Further progress in prefractionation procedures is expected to allow the detection of symbiotic proteins showing low abundance or being present in certain cell compartments.
year | journal | country | edition | language |
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2004-01-01 |