6533b834fe1ef96bd129ceb9
RESEARCH PRODUCT
Shuttling of the autoantigen La between nucleus and cell surface after uv irradiation of human keratinocytes.
Michael BachmannHanoch SlorWerner E. G. M�llerJörg KukuliesSooghee Changsubject
KeratinocytesCytoplasmTranscription GeneticUltraviolet RaysCellFluorescent Antibody TechniqueBiologyAutoantigensTranscription (biology)Epidermal growth factormedicineHumansNuclear proteinCell NucleusEpidermal Growth FactorCell MembraneBiological TransportCell BiologyCell biologyErbB ReceptorsCell nucleusmedicine.anatomical_structureBiochemistryRibonucleoproteinsCytoplasmProtein BiosynthesisKeratinocyteNucleusdescription
During the past years we have established that the nuclear autoantigen La shuttles between the nucleus and the cytoplasm in tumor cells after inhibition of transcription or virus infection. We reinvestigated this shuttling using primary human keratinocytes from both healthy donors and patients with xeroderma pigmentosum. Ultraviolet irradiation resulted in both an inhibition of transcription and a translocation of La protein from the nucleus to the cytoplasm. After a prolonged inhibition of transcription La protein relocated into the nucleus and assembled with nuclear storage regions. The uv-induced shuttling included a translocation to the cell surface, where La protein colocalized with epidermal growth factor receptors.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1990-12-01 | Experimental cell research |