6533b834fe1ef96bd129d5f5

RESEARCH PRODUCT

Characterization of three different clusters of 18S-26S ribosomal DNA genes in the sea urchin P. lividus: Genetic and epigenetic regulation synchronous to 5S rDNA.

Eufrosina DimarcoFabio CaradonnaDaniele Bellavia

subject

MSRE-PCR analysi0301 basic medicineMethylation statuSea urchinBiologyRibosomeDNA RibosomalParacentrotus lividusEpigenesis Genetic03 medical and health scienceschemistry.chemical_compound0302 clinical medicinebiology.animalSequence Homology Nucleic AcidGeneticsRNA Ribosomal 18SGene silencingAnimalsEpigeneticsGeneRibosomal DNASea urchinGeneticsBase SequenceRNA Ribosomal 5SGeneral MedicineSequence Analysis DNAbiology.organism_classificationRibosomeSettore BIO/18 - Genetica030104 developmental biologychemistryRNA Ribosomal030220 oncology & carcinogenesisMultigene Family18S-26S rDNAParacentrotusDNA

description

We previously reported the characterization 5S ribosomal DNA (rDNA) clusters in the common sea urchin Paracentrotus lividus and demonstrated the presence of DNA methylation-dependent silencing of embryo specific 5S rDNA cluster in adult tissue. In this work, we show genetic and epigenetic characterization of 18S-26S rDNA clusters in this specie. The results indicate the presence of three different 18S-26S rDNA clusters with different Non-Transcribed Spacer (NTS) regions that have different chromosomal localizations. Moreover, we show that the two largest clusters are hyper-methylated in the promoter-containing NTS regions in adult tissues, as in the 5S rDNA. These findings demonstrate an analogous epigenetic regulation in small and large rDNA clusters and support the logical synchronism in building ribosomes. In fact, all the ribosomal RNA genes must be synchronously and equally transcribed to perform their unique final product.

yearjournalcountryeditionlanguage
2015-04-30Gene
10.1016/j.gene.2016.01.010https://pubmed.ncbi.nlm.nih.gov/26789074