MRNS iepakošana in vivo no bakteriofāga GS atvasinātās vīrusiem līdzīgās daļiņās

6533b834fe1ef96bd129daff

RESEARCH PRODUCT

MRNS iepakošana in vivo no bakteriofāga GS atvasinātās vīrusiem līdzīgās daļiņās

Dagnija ĀRgule

subject

description

Šajā maģistra darbā ir parādīta zaļā fluorescentā proteīna (GFP) mRNS iepakošana in vivo Saccharomyces cerevisiae/pESC-URA ekspresijas sistēmā natīvās bakteriofāga GA apvalka proteīna veidotās vīrusiem līdzīgās daļiņās (VLD) un daļiņās, kurās apzināti ieviestas aminoskābju mutācijas. Tika pārbaudīta VLD veidošanās, apvalka proteīnā ieviešot aminoskābju mutācijas R43→N; K55→N vai S87→N un dubultmutācijas K55→N un S87→N vai S87→N un R43→N, un salīdzināts VLD ieguvums. VLD tika noteikta totālā RNS pakošanas kapacitāte un mRNS procentuālā daļa no tās. No VLD izdalītajās mRNS tika pierādītas GFP sekvence. Konstrukcijā, kurā aiz GFP gēna tika ievietota bakteriofāga MS2 operatora sekvence, tika pierādīts, ka VLD šāda mRNS tiek iepakota efektīvāk nekā gadījumos bez MS2 operatora sekvences. Atslēgvārdi: VLD, iepakošana in vivo, bakteriofāgs GA, GFP.

year	journal	country	edition	language
2013-01-01

https://dspace.lu.lv/dspace/handle/7/24946

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