6533b834fe1ef96bd129e03a
RESEARCH PRODUCT
Medium-size droplets of methyl ricinoleate are reduced by cell-surface activity in the gamma-decalactone production by Yarrowia lipolytica.
Jean-marc NicaudJean-marc BelinK. BergmarkY. WachéMario AguedoJ.-l. Courthaudonsubject
0106 biological sciences[SDV.BIO]Life Sciences [q-bio]/BiotechnologyTime FactorsCell01 natural sciencesApplied Microbiology and BiotechnologyLactonesBiotransformationMESH : Particle SizeYeastsMESH: Microscopy Confocal[INFO.INFO-BT]Computer Science [cs]/BiotechnologyComputingMilieux_MISCELLANEOUSBiotransformation0303 health sciencesMicroscopyMicroscopy ConfocalbiologyMESH: YeastsMESH : Lactones[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitologymedicine.anatomical_structureBiochemistryConfocalSURFACE ACTIVERicinoleic Acids[ INFO.INFO-BT ] Computer Science [cs]/BiotechnologyMESH: LactonesMESH : Time Factors03 medical and health sciencesMESH : Biotransformation010608 biotechnologymedicine[SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyMESH: Particle SizeParticle SizeMESH : Microscopy Confocal[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular BiologyMethyl ricinoleateMESH: BiotransformationMESH : YeastsChromatography030306 microbiologyMESH: Time Factors[ SDV.BIO ] Life Sciences [q-bio]/BiotechnologyYarrowiabiology.organism_classificationYeastMESH: Ricinoleic AcidsCulture Media[SDV.BIO] Life Sciences [q-bio]/Biotechnology[INFO.INFO-BT] Computer Science [cs]/BiotechnologyMESH : Ricinoleic AcidsMESH: Culture MediaMESH : Culture Mediadescription
International audience; Size of methyl ricinoleate droplets during biotransformation into gamma-decalactone by Yarrowia lipolytica was measured in both homogenized and non-homogenized media. In non-homogenized but shaken medium, droplets had an average volume surface diameter d32 of 2.5 microm whereas it was 0.7 microm in homogenized and shaken medium. But as soon as yeast cells were inoculated, both diameters became similar at about 0.7 microm and did not vary significantly until the end of the culture. The growth of Y. lipolytica in both media was very similar except for the lag phase which was lowered in homogenized medium conditions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-03-01 |