6533b835fe1ef96bd129ec44

RESEARCH PRODUCT

Transcription of human neuronal nitric oxide synthase mRNAs derived from different first exons is partly controlled by exon 1-specific promoter sequences

Jean-paul BoisselMatthias BrosUte Gödtel-armbrustUlrich Förstermann

subject

Transcription Genetic5' Flanking Region5' flanking regionReporter gene assaysSkeletal muscleNitric Oxide Synthase Type IBiologyKidneyHippocampusCell LineRT real-time PCRExonExon trappingGenes ReporterTestisGeneticsHumansRNA MessengerCloning MolecularLuciferasesPromoter Regions GeneticGeneSkinBinding SitesSplice site mutationReverse Transcriptase Polymerase Chain ReactionAlternative splicingGenetic VariationHeartExonsMolecular biologyAlternative SplicingRNA splicingCortexTandem exon duplicationProtein BindingTranscription Factors

description

AbstractThe human neuronal nitric oxide synthase (NOS1) gene is subject to extensive splicing. A total of 12 NOS1 mRNA species have been identified. They differ in their 5′ ends and are derived from 12 different first exons (termed exons 1a to 1l). Various cell lines whose NOS1 first exon expression patterns were representative of human brain, skin, and skeletal muscle were identified. These included A673 neuroepithelioma cells, SK-N-MC neuroblastoma cells, HaCaT keratinocyte-like cells, and C2C12 myocyte-like cells. In these cell lines, correlations were found between the exon 1 variants preferentially expressed and the promoter activities of their cognate 5′ flanking sequences. These data demonstrate that expression of the different exon 1-related splice variants of NOS1 mRNA is controlled directly (at least in part) by the associated 5′ flanking sequences.

yearjournalcountryeditionlanguage
2006-04-01Genomics
https://doi.org/10.1016/j.ygeno.2005.11.013