6533b835fe1ef96bd129f2ce
RESEARCH PRODUCT
Polymer support synthesis
J.n. KinkelUdo KotschiHartmut SelignerCaroline ScharpfFriedhelm EisenbeissKlaus K. UngerRainer Martinsubject
chemistry.chemical_classificationChromatographyOligonucleotideSilica gelOrganic ChemistryGeneral MedicinePolymerengineering.materialOligonucleotide synthesisBiochemistryAnalytical Chemistrychemistry.chemical_compoundChain lengthchemistryCoatingPolymer chemistryengineeringElongationPorositydescription
Non-porous silica gel microbeads of diameter 1.5 microns have been investigated as supports for oligonucleotide synthesis. In the preparation of oligothymidylates of chain length up to 150 bases, with 5'-di-p-anisylphenylmethyl-3'-phosphoramidite as an intermediate, the average yields per chain elongation were up to 99%. Lower overall yields were observed in the case of a support which developed a strong tendency towards aggregation after the build up of an oligonucleotide coating.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1989-01-01 | Journal of Chromatography A |