New adsorbents for thymidylate synthase affinity chromatography

6533b835fe1ef96bd129f3b0

RESEARCH PRODUCT

New adsorbents for thymidylate synthase affinity chromatography

Leszek Krzyzanowski Krzysztof Pawelczak Joanna Ciesla Piotr Wieczorek Michal Kempny Barbara Rzeszotarska Wojciech Rode

subject

Adsorption Chromatography Affinity chromatography biology Chemistry biology.protein General Chemistry Thymidylate synthase

description

New affinity adsorbents, intended for chromatography of thymidylate synthase (EC 2.1.1.45) from different sources, consisting of p-[N-[(2-amino-4-hydroxy-6-quinazolinyl)-methyl]-N-2-propynylamino]benzoyl-γ-[α-(3-carboxypropylamino)]glutamyl-glutamyl immobilized either on macroporous copolymer of acrylonitrile and n-butyl acrylate or on macroporous polymer of acrylonitrile itself, both crosslinked with divinylbenzene and having aminoethyl groups, were obtained. Both adsorbents were found to be effective in dUMP-dependent binding of thymidylate synthase from regenerating rat liver.

year	journal	country	edition	language
1988-01-01	Collection of Czechoslovak Chemical Communications

https://doi.org/10.1135/cccc19882890

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