6533b835fe1ef96bd129f3d6
RESEARCH PRODUCT
Mannoproteins from the cell wall ofKluyveromyces lactis
Enrique HerreroAlan Boydsubject
Kluyveromyces lactisAntiserumbiologySaccharomyces cerevisiaebiology.organism_classificationMicrobiologyYeastDithiothreitolCell wallchemistry.chemical_compoundchemistryBiochemistryKluyveromycesGeneticsSodium dodecyl sulfateMolecular Biologydescription
Wall mannoproteins from Kluyveromyces lactis have been solubilised by treatment of cell walls with sodium dodecyl sulphate (SDS) or zymolyase. While the former reagent liberates a large number of molecular species, zymolyase preferentially releases a high-molecular-weight material that is sensitive to endo-β-N-acetylglucosaminidase H, and a 29-kDa molecule that reacts with the antiserum raised against a similar species from walls of Saccharomyces cerevisiae. In contrast with observations on isolated walls of S. cerevisiae, dithiothreitol pretreatment of K. lactis walls does not enhance the effect of zymolyase upon mannoprotein release. However, the action of thiol agents is still necessary to obtain protoplasts by zymolyase digestion from K. lactis whole cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1986-09-01 | FEMS Microbiology Letters |