6533b835fe1ef96bd129f5b0

RESEARCH PRODUCT

A homemade device for linear sucrose gradients

Giorgia SisinoRainer BarbieriDoriana CelluraDaniele Bellavia

subject

centrifugationChromatographySucroseBiophysicsAnalytical chemistryReproducibility of ResultsLinearityCell BiologySucrose gradientsucrose gradientBiochemistryLinear gradientchemistry.chemical_compoundchemistryCentrifugation Density GradientCosts and Cost AnalysisTube (container)LaboratoriesMolecular BiologyRefractive indexlinear gradient

description

We have developed a simple and inexpensive device to obtain linear sucrose gradients with commonly used laboratory materials--a syringe, a flask, a plastic tube, and a piece of Pongo (Play-Doh). Refractive index values measured on sucrose fractions collected using our system demonstrate both the linearity and reliability of the gradients obtained.

yearjournalcountryeditionlanguage
2008-04-08Analytical Biochemistry
https://doi.org/10.1016/j.ab.2008.05.010