6533b835fe1ef96bd129f627

RESEARCH PRODUCT

Single-Spore Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi

Dirk Redecker

subject

0106 biological sciences0301 basic medicineGenotypingbiologyObligatefungiExtraction (chemistry)Single-spore extractionBiomassArbuscular mycorrhizal fungibiology.organism_classification01 natural sciencesSporeGlomeromycota03 medical and health sciences030104 developmental biology[SDE]Environmental SciencesBotanyGlomeromycota010606 plant biology & botany

description

International audience; Biomass of arbuscular mycorrhizal fungi (AMF, Glomeromycota) is often only available in small quantities as these fungi are obligate biotrophs and many species are difficult to cultivate under controlled conditions. Here, I describe a simple, efficient approach to produce crude extracts from single or a small number of spores that can be used for genotyping AMF.

yearjournalcountryeditionlanguage
2020-09-08
https://doi.org/10.1007/978-1-0716-0603-2_8