6533b835fe1ef96bd129fcf3

RESEARCH PRODUCT

SINGLE-PEAK RESOLUTION CRITERIA FOR OPTIMIZATION OF MOBILE PHASE COMPOSITION IN LIQUID CHROMATOGRAPHY

subject

description

Three criteria that evaluate the single peak resolution (peak purity) in chromatography, the free height fraction, free area fraction, and valley ratio, are examined. The main advantages of these criteria against other criteria based on peak pair separation, are that the measurements are not affected by the identity of neighboring peaks and are normalized, which make them very intuitive. The methodology is illustrated through the isocratic separation of mixtures of several sulphonamides (sulphacetamide, sulphadiazine, sulphadimethoxine, sulphaguanidine, sulphamerazine, sulphamethazine, sulphamethoxazole, sulphamethizole, sulphamonomethoxine, sulphanilamide, sulphapyridine, sulphaquinoxaline, and sulphathiazole). Among the three proposed criteria, the measurement of free area fractions gave the best description of the resolution, independent of the overlapping degree of the chromatographic peaks. The usefulness of this criterion for the resolution of individual peaks in a complex mixture is shown. The best...