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RESEARCH PRODUCT

Additional file 1 of miR-486-5p expression is regulated by DNA methylation in osteosarcoma

Heidi M. Naml��sMagne Sk��rnDeeqa AhmedIwona GradKim AndresenStine H. KresseElse MuntheMassimo SerraKatia ScotlandiAntonio Llombart-boschOla MyklebostGuro E. LindLeonardo A. Meza-zepeda

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Additional file 1: Supplementary Table S1. Overview of TaqMan assays for miRNA and mRNA qRT-PCR experiments. Supplementary Table S2. Primers used for qualitative Methylation-Specific Polymerase Chain Reaction (MSP), bisulfite sequencing (BS) and quantitative Methylation-Specific Polymerase Chain Reaction (qMSP). Supplementary Figure S1. Genomic overview of the mir-486/ANK1 locus. Supplementary Figure S2. Expression of ANK1 transcript variants in untreated and 5-Aza treated osteosarcoma cell lines. Supplementary Figure S3. Methylation level of miR-486 as assessed by methylation specific PCR (MSP).

https://dx.doi.org/10.6084/m9.figshare.19185704.v1