6533b835fe1ef96bd12a004c
RESEARCH PRODUCT
Two tyrosinase nonapeptides recognized on HLA-A2 melanomas by autologous cytolytic T lymphocytes
Barbara SeligerThomas WölfelV BrichardK H Meyer Zum BüschenfeldeJörg SchneiderThierry BoonA Van Pelsubject
Cytotoxicity ImmunologicSignal peptideTyrosinaseMolecular Sequence DataImmunologyClone (cell biology)Tyrosinase PeptidePeptideIn Vitro TechniquesBiologyHLA-A2 AntigenTumor Cells CulturedConsensus sequenceHumansImmunology and AllergyAmino Acid SequenceMelanomaPeptide sequenceDNA Primerschemistry.chemical_classificationBase SequenceMonophenol MonooxygenaseVirologyRecombinant ProteinsCTL*chemistryPeptidesT-Lymphocytes Cytotoxicdescription
A number of cytolytic T lymphocyte (CTL) clones derived from several melanoma patients have been found to recognize a majority of melanomas from HLA-A2 patients. We have reported previously that two such CTL clones recognize a product of the tyrosinase gene that is presented by HLA-A2. Here we show that one of these CTL clones recognizes a peptide encoded by the first nine amino acids of the putative signal sequence of tyrosinase. The other CTL clone recognizes a different tyrosinase peptide corresponding to amino acids 368-376. Both peptides contain consensus motifs of HLA-A2 binding peptides.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-03-01 | European Journal of Immunology |