Laboratorijas metožu aprobācija lauka pupu izturības pret lapu patogēniem Botrytis fabae, Ascochyta fabae un Stemphylium sarcinaeforme paātrinātai noteikšanai

6533b836fe1ef96bd12a03a6

RESEARCH PRODUCT

Laboratorijas metožu aprobācija lauka pupu izturības pret lapu patogēniem Botrytis fabae, Ascochyta fabae un Stemphylium sarcinaeforme paātrinātai noteikšanai

Elīna Anna Brauna

subject

slimības skrīninga metode Lauku pupas Bioloģija šķirņu izturība

description

Darba mērķis bija salīdzināt 15 lauku pupu šķirņu izturību pret 2015. gada veģetācijas sezonas nozīmīgākajiem lauka pupu lapu slimību ierosinātājiem – Botrytis fabae, Ascochyta fabae un Stemphylium sarcinaeforme, izmantojot laboratorijas skrīninga metodi, un salīdzināt iegūtos rezultātus ar lauka šķirņu salīdzinājuma izmēģinājuma rezultātiem. Pēc iegūtajiem rezultātiem secināt, vai izmantotā laboratorijas skrīniga metode var tikt pielietota paātrinātai šķirņu izturības noteikšanai pret šiem patogēniem. Laboratorijas skrīninga eksperimentā izmantoja lapu pilienu testu. Nogrieztas pupu lapas inficēja ar patogēna sporu suspensiju pilieniem. Pēc simptomu parādīšanās, piecu dienu laikā mērīja slimības izraisītā plankuma diametru. Skrīninga eksperimenta rezultāti liecina, ka lauka pupu šķirnes būtiski atšķīrās pēc ieņēmības pret B. fabae un S. sarcinaeforme un šo metodi var izmantot lauka pupu selekcijas procesā paātrinātai šķirņu pārbaudei. A. fabae izraisītie plankumi attīstījās nevienmērīgi un uz vismaz 75% šķirņu lapu slimības simptomi neparādījās vispār. Šķirņu ieņēmības salīdzinājumus pret A. fabae nepieciešams turpināt, pārbaudot citas laboratorijas metodes.

year	journal	country	edition	language
2016-01-01

https://dspace.lu.lv/dspace/handle/7/32204

PEPhysical Sciences and Engineering
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