6533b836fe1ef96bd12a0824

RESEARCH PRODUCT

Physiological, morphological and metabolic changes in Tetrahymena pyriformis for the in vivo cytotoxicity assessment of metallic pollution: Impact on d-β-hydroxybutyrate dehydrogenase

Norbert LatruffeDriss MountassifM'hammed Saïd El KebbajNoureddine BourhimMostafa KabineZaina ZaroualRachid Manar

subject

chemistry.chemical_classificationEcologyGeneral Decision SciencesDehydrogenaseMitochondrionCadmium chlorideBiologychemistry.chemical_compoundEnzymechemistryBiochemistryIn vivoTetrahymena pyriformisInner mitochondrial membraneCytotoxicityEcology Evolution Behavior and Systematics

description

Abstract The individual cytotoxicity of cadmium chloride, iron sulphate and chromium nitrate has been investigated by using the freshwater ciliate Tetrahymena pyriformis. The metabolic enzymes and antioxidant defense biomarkers were assessed. The results obtained reveal that their metal salts have perturbed the physiology and morphology of T. pyriformis. Also, the biomarkers assessed were sensitive to the presence of metal salts and this sensitivity was metal salt and dose dependant. To estimate the impact of their metal salts on mitochondria, we studied their effects in vivo and in vitro on the d -β-hydroxybutyrate dehydrogenase (BDH) (EC 1.1.1.30) inner mitochondrial membrane enzyme. The results showed a high inhibition of BDH in terms of activity, protein expression and kinetic parameters.

yearjournalcountryeditionlanguage
2007-11-01Ecological Indicators
https://doi.org/10.1016/j.ecolind.2006.11.010