6533b836fe1ef96bd12a0b83

RESEARCH PRODUCT

Polymeric monolithic microcartridges with gold nanoparticles for the analysis of protein biomarkers by on-line solid-phase extraction capillary electrophoresis-mass spectrometry

José Manuel Herrero-martínezLaura PontVictoria Sanz-nebotLeonardo Gabriel GagliardiMaría Vergara-barberánFernando BenaventeGemma Marin

subject

MASS SPECTROMETRYPolymersMetal NanoparticlesOr010402 general chemistryMass spectrometry01 natural sciencesBiochemistryCapillary electrophoresis–mass spectrometryMass SpectrometryAnalytical Chemistry//purl.org/becyt/ford/1 [https]Capillary electrophoresisCAPILLARY ELECTROPHORESISLimit of DetectionElectroforesi capil·lar//purl.org/becyt/ford/1.4 [https]HumansPrealbuminSolid phase extractionChromatographyNanopartículesChemistryElutionSolid Phase Extraction010401 analytical chemistryOrganic ChemistryON-LINE SOLID-PHASE EXTRACTIONElectrophoresis CapillaryReproducibility of ResultsGeneral MedicineMONOLITHIC MICROCARTRIDGETRANSTHYRETIN0104 chemical sciencesEspectrometria de massesIsoelectric pointColloidal goldGOLD NANOPARTICLESEpoxy CompoundsMethacrylatesNanoparticlesGoldIon trapBiomarkers

description

In this study, polymeric monoliths with gold nanoparticles (AuNP@monolith) were investigated as microcartridges for the analysis of protein biomarkers by on-line solid-phase extraction capillary electrophoresis-mass spectrometry (SPE-CE-MS). “Plug-and-play” microcartridges (7 mm) were prepared from a glycidyl methacrylate (GMA)-based monolithic capillary column (5 cm x 250 µm i.d.), which was modified with ammonia and subsequently functionalized with gold nanoparticles (AuNPs). The performance of these novel microcartridges was evaluated with human transthyretin (TTR), which is a protein related to different types of familial amyloidotic polyneuropathies (FAP). Protein retention depended on the isoelectric point of the protein (TTR pI~5.4) and elution was achieved with a basic phosphate solution. Under the optimized conditions, limits of detection (LODs) for TTR by AuNP@monolith-SPE-CE-MS were 50 times lower than by CE-MS (5 vs 250 mg•L−1, with an ion trap (IT) mass spectrometer). The sensitivity enhancement was similar compared to SPE-CE-MS using immunoaffinity (IA) microcartridges with intact antibodies against TTR. Linearity, repeatability in migration times and peak areas, reusability, reproducibility and application to serum samples were also evaluated. Fil: Pont, Laura. Universidad de Barcelona; España Fil: Marin, Gemma. Universidad de Barcelona; España Fil: Vergara Barberán, María. Universidad de Valencia; España Fil: Gagliardi, Leonardo Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Sanz Nebot, Victoria. Universidad de Barcelona; España Fil: Herrero Martinez, José Manuel. Universidad de Valencia; España Fil: Benavente, Fernando. Universidad de Barcelona; España

yearjournalcountryeditionlanguage
2020-07-05
10.1016/j.chroma.2020.461097https://www.sciencedirect.com/science/article/abs/pii/S0021967320303186