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RESEARCH PRODUCT
Variation of proteomic profile during lactation in Girgentana goat milk: a preliminary study
Giuseppina MonteleoneRosalia Di GerlandoSalvatore MastrangeloAnna Maria SuteraBaldassare PortolanoMaria Teresa SardinaMarco Tolonesubject
Globulin040301 veterinary sciencesGoat milk proteome 2D-DIGE Girgentana breed2D-DIGE; Girgentana breed; Goat milk proteome; Animal Science and ZoologyProteomics0403 veterinary scienceSettore AGR/17 - Zootecnica Generale E Miglioramento GeneticoLactationCaseinGirgentana breedmedicineGoat milk proteomeFood scienceBeta-lactoglobulinlcsh:SF1-1100Proteomic Profilebiology0402 animal and dairy science04 agricultural and veterinary sciences040201 dairy & animal scienceBreedmedicine.anatomical_structureProteomebiology.protein2D-DIGEAnimal Science and Zoologylcsh:Animal culturedescription
The knowledge of milk proteome has been greatly enhanced by technological advances in the proteomics field as the use of the two-dimensional differential in-gel electrophoresis, a gel-based approach which allowed the analysis of proteins from complex mixtures and the comparing of several protein samples in the same experiment. The aim of this study was to characterise the whole milk proteomic profile in Girgentana dairy goat breed by two-dimensional differential in-gel elecrophoresis. The obtained representative 2D whole milk proteomic map showed a general picture of the protein distributions over the pH 3–10 NL including about 100 spots, most of them organised like a spot train. Among differentially abundant spots in the three experimental conditions, milk fat globule EGF factor 8 protein, β-lactoglobulin, β-casein and serum albumin were successfully identified. The three-dyes system employed in two-dimensional differential in-gel electrophoresis (2D-DIGE) analysis allowed us to obtain a global representation of the Girgentana whole milk proteome. These preliminary results could be used to generate a milk reference proteomics map for the Girgentana goat breed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-01-01 |