Sekundāro metabolītu izdalīšana no sūkļa Smenospongia aurea

6533b836fe1ef96bd12a0e11

RESEARCH PRODUCT

Sekundāro metabolītu izdalīšana no sūkļa Smenospongia aurea

subject

Smenospongia aurea AEŠH jūras izcelsmes dabas produkti Farmācija 1H KMR NRP/PK

description

Darba mērķis bija jaunu sekundāro metabolītu izolēšana no sūkļa Smenospongia aurea un to ķīmiskās struktūras noteikšana. Tomēr, uzsākot darbu, nolēmām izdalīt oriģinālus, neribosomālus hibrīdu peptīdu/poliketīdu savienojumus – smenamīdu A, smenamīdu B, smenotiazolu A un smenotiazolu B –, kas jau iepriekš izdalīti no S. aurea sūkļa un kam noteikta augsta citotoksiska aktivitāte. Interesējošos savienojumus izdalījām no lipofīla sūkļa S. aurea ekstrakta, izmantojot augsti efektīvo šķidrumu hromatogrāfijas (AEŠH) metodi, to ķīmisko struktūru noteicām ar augstas izšķirtspējas masspektrometrijas (ESI/MS), 1H kodolu magnētiskās rezonanses (KMR) un 13C KMR metodēm, un iegūtos datus salīdzinājām ar literatūras datiem. Rezultātā izdevās izdalīt individuālus savienojumus – smenamīdu A, smenamīdu B, smenotiazolu A un smenotiazolu B – ļoti mazos daudzumus, ko var skaidrot ar bromēto alkaloīdu daudzumu S. aurea sūklī, kas apgrūtina sekundāro metabolītu hromatogrāfisku atdalīšanu.

year	journal	country	edition	language
2015-01-01

https://dspace.lu.lv/dspace/handle/7/30845

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