6533b836fe1ef96bd12a127c

RESEARCH PRODUCT

Purification and identification of chlorophyll c1 from the green alga Mantoniella squamata

subject

description

Abstract The prasinophycean alga Mantoniella squamata contains besides chlorophyll a and b a third chlorophyll c -like pigment in its light-harvesting antenna. This third chlorophyll was purified by reverse phase and polyethylene chromatography in order to identify its chemical structure. The absorption and fluorescence spectra were measured not only from the doubly purified pigment, but also from its Mg-free derivates. The spectra were compared with those of authentic chlorophyll c and of Mg-2,4-desethyl-2,4-divinylpheoporphyrin a 5 monomethyl ester which was isolated from Rhodobacter capsulata . The results show that the pigment from Mantoniella agrees best with chlorophyll c 1 . In order to clarify the spectral data, chlorophyll c 1 and c 2 , the pigment from Mantoniella and Mg-2,4-desethyl-2,4-divinylpheoporphyrin a 5 monomethyl ester were resolved by polyethylene chromatography. The chromatographic analysis clearly shows that the pigment from Mantoniella comigrates with chlorophyll c 1 and not with the bacterial pigment or chlorophyll c 2 . Mantoniella is the first organism which has been demonstrated to contain chlorophyll a , b and c .