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RESEARCH PRODUCT
Detection of Human Immunodeficiency Virus-1 Nucleic Acid on Inactivated Filter Paper Disks by Polymerase Chain Reaction and Microtiter Plate Assay
Wolfgang RöderKunihiro SaitoWerner E. G. M�llerHiroshi UshijimaMichael KruseShuji AndoTakao KunisadaYuki Eshitasubject
PaperHot TemperatureMolecular Sequence DataImmunologyHIV InfectionsBiologyPolymerase Chain ReactionSensitivity and SpecificityMicrobiologyViruslaw.inventionImmunoenzyme Techniqueschemistry.chemical_compoundMicrotiter platelawVirologyHumansFalse Positive ReactionsCells CulturedPolymerase chain reactionBase SequenceFilter paperRNAGenes gagMolecular biologychemistryDNA ViralHIV-1Nucleic acidRNA ViralNested polymerase chain reactionFiltrationDNAdescription
Human immunodeficiency virus type 1 (HIV-1) in cultured cells, peripheral blood samples and sera were adsorbed on filter paper disks and inactivated by heat or ethanol. Two procedures, the polymerase chain reaction (PCR) and microtiter plate assay (HMPA) were used to detect the nucleic acid. The sensitivity after different heat treatments with nested PCR for HIV-1 DNA (or nested reverse transcription-PCR for HIV-1 RNA) was identical regardless of whether the samples were examined immediately or one month later. Inactivation by ethanol treatment resulted in a slight loss of sensitivity. The HMPA proved to be as reliable and specific as the conventional PCR technique. We conclude that the heat-treated filter paper disk assay is suitable for identifying HIV nucleic acid in clinical samples sent to the laboratory from a distance, e.g. in an envelope.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-08-01 | Microbiology and Immunology |