6533b836fe1ef96bd12a1b95

RESEARCH PRODUCT

false

C.a. YeatesA.l. BotesHenning M. KriegJ MaritzJaco C. Breytenbach

subject

chemistry.chemical_classificationCalcium alginateChromatographybiologyKineticsRhodosporidium toruloideschemistry.chemical_elementBioengineeringGeneral MedicineCalciumbiology.organism_classificationApplied Microbiology and BiotechnologyEnzyme assayYeastKinetic resolutionchemistry.chemical_compoundEnzymechemistryBiochemistrybiology.proteinBiotechnology

description

Resting cells of the yeast Rhodosporidium toruloides (UOFS Y-0471) were immobilised in calcium alginate beads for the enantioselective kinetic resolution of racemic-1,2-epoxyoctane. The initial activity exhibited by immobilised cells was almost 50% lower than that of the free counterpart but was extremely stable when compared to the free cells. The concentration of the immobilised biomass had no effect on apparent enzyme activity but did lead to a decrease in single cell activity. An increase in both the alginate and CaCl2 concentrations used for bead preparation led to a decrease in enzyme stability. An increase in the alginate concentration led to an increase in bead diameter. The stoichiometric equation for cross-linking of alginate was only obeyed when CaCl2 concentrations higher than 0.4 M were utilised for bead preparation.

yearjournalcountryeditionlanguage
2003-01-01Biotechnology Letters
https://doi.org/10.1023/a:1026044113856