6533b837fe1ef96bd12a1de3

RESEARCH PRODUCT

Transglutaminase and polyamine dependence of effector functions of human immunocompetent cells

Hartmut Hanauske-abelRudolf E. SchopfHermann Schulte-wissermannVolkmar Günzler

subject

Dansyl cadaverineDNA ReplicationPolyamineLuminescenceMitoguazoneTissue transglutaminaseLymphocyteBiophysicsStimulationLymphocyte proliferationDiaminesGranulocyteBiologyLymphocyte ActivationGuanidinesBiochemistrylaw.inventionMeGbGchemistry.chemical_compoundStructural BiologylawCadaverinePolyaminesGeneticsmedicineHumansLymphocytesMolecular BiologyChemiluminescenceCadaverineTransglutaminasesGranulocyteCell BiologyTransglutaminaseMolecular biologyKineticsmedicine.anatomical_structurechemistryBiochemistrybiology.proteinLymphocytePolyamineAcyltransferasesGranulocytes

description

AbstractThe effects of the transglutaminase inhibitor dansyl cadaverine (DC) and the polyamine antagonist methyl glyoxal-bis-(guanylhydrazone) (MeGbG) on the response of lymphocytes towards allogeneic and lectin stimulation and on the zymosan-induced chemiluminescence of neutrophilic granulocytes was studied. Application of DC resulted in dose-dependent suppression of chemiluminiscence and lymphocyte proliferation; no difference of inhibitory potential occurred with variation of incubation time in the latter system. MeGbG was inactive in granulocytes, but inhibited lymphocyte proliferation; its effect increased with time. The experiments provide further evidence for the importance of transglutaminases and polyamines for the function of immunocompetent cells.

yearjournalcountryeditionlanguage
1982-12-27FEBS Letters
https://doi.org/10.1016/0014-5793(82)80774-6