6533b837fe1ef96bd12a1eac
RESEARCH PRODUCT
Monitoring WWTP performance using size-exclusion chromatography with simultaneous UV and fluorescence detection to track recalcitrant wastewater fractions
Tuula TuhkanenA. N. Ignat’evsubject
Environmental EngineeringHealth Toxicology and Mutagenesis0208 environmental biotechnologySize-exclusion chromatographyta117202 engineering and technologyjätevesiWastewater010501 environmental sciences01 natural sciencesHigh-performance liquid chromatographyWater Purificationwastewater characterizationWater SupplyEnvironmental ChemistryOrganic ChemicalsEffluentChromatography High Pressure LiquidFinlandActivated sludge system0105 earth and related environmental sciencesjäteveden käsittelyChromatographyChemistryPublic Health Environmental and Occupational HealthUV absorptionGeneral MedicineGeneral Chemistrymolecular weight distributionPollutionFluorescence020801 environmental engineeringsize-exclusion chromatographywastewater treatmentSpectrometry FluorescenceWastewaterChromatography GelMolar mass distributionSpectrophotometry UltravioletSewage treatmentfluorescenceWater Pollutants ChemicalEnvironmental Monitoringdescription
Abstract A trial monitoring of a typical full-scale municipal WWTP in Central Finland was aimed to explore applicability of high performance liquid chromatography – size exclusion chromatography (HPSEC) with simultaneous UV and fluorescence detection as a tool for advanced routine monitoring of wastewater treatment. High, intermediate, and low molecular weight (MW) fractions of untreated wastewater (influent) and treated wastewater (secondary effluent) were characterized in terms of UV absorbance at 254 nm (UVA254) and specific fluorescence representing tyrosine-like, tryptophan-like, and humic/fulvic-like compounds. The activated sludge treatment removed 97 ± 1% of BOD, 93 ± 2% of COD, 71 ± 7% of DOC, and 24 ± 7% of TN, while the overall reduction of UVA254 was 50 ± 6%. Total fluorescence signal declined by ∼80% for tyrosine-like, by 60–70% for tryptophan-like, and by 7–36% for humic/fulvic-like compounds. Low and intermediate MW humic/fulvic-like compounds fluorescing at λex/λem = 390/500 nm demonstrated recalcitrant behavior. Protein-like and humic/fulvic-like fractions of low MW
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2018-04-21 | Chemosphere |