6533b837fe1ef96bd12a1f14
RESEARCH PRODUCT
Macromolecules in ordered media: 7. Influence of ionic strength and bilayer composition on the association of polyelectrolytes to mixed liposomes
Rosa GarcíaConcepción AbadClara M. GómezAgustín CamposIolanda Porcarsubject
LiposomePolymers and PlasticsBilayerVesicleOrganic Chemistrytechnology industry and agriculturePhospholipidElectrolytePolyelectrolytechemistry.chemical_compoundchemistryChemical engineeringIonic strengthPhosphatidylcholineMaterials ChemistryOrganic chemistrylipids (amino acids peptides and proteins)description
The effect of both the phospholipid composition of the bilayer and the ionic strength of the medium on the association between poly(2-vinyl pyridine) and mixed liposomes based on dimyristoyl phosphatidic acid and dimyristoyl phosphatidil choline was investigated using fluorescence spectroscopy. Intensity changes upon addition of vesicles have allowed us to estimate the extent of the association and data have been processed as association isotherms. We found that the association is enhanced by increasing both the ionic strength and the anionic phospholipid fraction. However, whereas the negative net charge of the bilayer strongly enhances the interaction, the presence of more and more electrolyte in the aqueous media induces a slight increase of the binding. Moreover, surfaces with at least 50% of charged phospholipid are necessary. Results were consistent with those obtained in preceding reports.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-09-01 | Polymer |