Mezenhimālo cilmes šūnu diferenciācijas noteikšana ar beziezīmes metodēm

6533b837fe1ef96bd12a21fc

RESEARCH PRODUCT

Mezenhimālo cilmes šūnu diferenciācijas noteikšana ar beziezīmes metodēm

Rebeka Rožkalne

subject

adipodiferenciācija attēlveidojošais MCŠ senescene Farmācija plūsmas

description

Maģistra darba mērķis bija veikt ādas mezenhimālo cilmes šūnu (MCŠ) diferenciāciju un noteikt ar to saistītās morfoloģiskās izmaiņas izmantojot attēlveidojošo plūsmas citometriju. Izmantojām šo metodi, lai raksturotu un atšķirtu šūnas bez iezīmēm, kas kultivētas dažādos apstākļos (audzētas sferoīdos, diferencētas par adipocītiem, kultivētas doksorubicīna klātbūtnē). Morfoloģiskie dati tika analizēti, izmantojot programmatūru “IDEAS”. Rezultāti parādīja, ka adipodiferencētās šūnas var atšķirties ne tikai pēc lieluma, bet arī pēc spilgtā lauka signāla intensitātes un tekstūras parametriem. 2D un 3D MCŠ ir atdalāmas ar dažādiem tumšā lauka kanāla parametriem. Senescentās šūnas var atšķirt no kontroles šūnām. Atšķirības novērojamas arī atkarībā no senescenci izraisošā doksorubicīna koncentrācijas un kultivēšanas ilguma.

year	journal	country	edition	language
2019-01-01

https://dspace.lu.lv/dspace/handle/7/48699

PEPhysical Sciences and Engineering
- PE2Fundamental Constituents of Matter
  - PE2_1Theory of fundamental interactions
  - PE2_2 Phenomenology of fundamental interactions
  - PE2_3Experimental particle physics with accelerators
  - PE2_4Experimental particle physics without accelerators
  - PE2_6Nuclear, hadron and heavy ion physics
  - PE2_8Gas and plasma physics
  - PE2_5Classical and quantum physics of gravitational interactions
  - PE2_7Nuclear and particle astrophysics
  - PE2_9 Electromagnetism
  - PE2_10Atomic, molecular physics
  - PE2_11Ultra-cold atoms and molecules
  - PE2_18Equilibrium and non-equilibrium statistical mechanics: steady states and dynamics
  - PE2_14Lasers, ultra-short lasers and laser physics
  - PE2_17Metrology and measurement
  - PE2_16Non-linear physics
  - PE2_12Optics, non-linear optics and nano-optics
  - PE2_13Quantum optics and quantum information
  - PE2_15Thermodynamics
- PE1Mathematics
  - PE1_1Logic and foundations
  - PE1_6Geometry and global analysis
  - PE1_8Analysis
  - PE1_7Topology
  - PE1_9Operator algebras and functional analysis
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  - PE1_12Mathematical physics
  - PE1_11Theoretical aspects of partial differential equations
  - PE1_13Probability
  - PE1_14Mathematical statistics
  - PE1_15Generic statistical methodology and modelling
  - PE1_4 Algebraic and complex geometry
  - PE1_19Scientific computing and data processing
  - PE1_2Algebra
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  - PE1_21Application of mathematics in sciences
  - PE1_22Application of mathematics in industry and society
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  - PE1_17Mathematical aspects of computer science
  - PE1_3Number theory
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  - PE3_1Structure of solids, material growth and characterisation
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  - PE3_3Transport properties of condensed matter
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  - PE3_6Macroscopic quantum phenomena, e.g. superconductivity, superfluidity, quantum Hall effect
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  - PE10_14Earth observations from space/remote sensing
  - PE10_11Geochemistry, cosmochemistry, crystal chemistry, isotope geochemistry, thermodynamics
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- PE11Materials Engineering
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  - PE11_7Engineering of metal oxides
  - PE11_8Engineering of alternative established or emergent materials
  - PE11_9Nanomaterials engineering, e.g. nanoparticles, nanoporous materials, 1D & 2D nanomaterials
  - PE11_10Soft materials engineering, e.g. gels, foams, colloids
  - PE11_11Porous materials engineering, e.g. covalent-organic, metal-organic, porous aromatic frameworks
  - PE11_12Semi-conducting and magnetic materials engineering
  - PE11_13Metamaterials engineering
  - PE11_14Computational methods for materials engineering
LSLife Sciences
- LS1Molecules of Life: Biological Mechanisms, Structures and Functions
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  - LS1_5Lipid biology
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  - LS1_11Chemical biology
  - LS1_12Protein design
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  - LS1_14Innovative methods and modelling in molecular, structural and synthetic biology
  - LS1_10Synthetic biology
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  - LS2_3Epigenetics
  - LS2_2Gene editing
  - LS2_4Gene regulation
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  - LS2_6Metagenomics
  - LS2_7Transcriptomics
  - LS2_8Proteomics
  - LS2_9Metabolomics
  - LS2_10Glycomics/Lipidomics
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  - LS2_12Biostatistics
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  - LS2_16Innovative methods and modelling in integrative biology
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- LS4Physiology in Health, Disease and Ageing
  - LS4_1Organ and tissue physiology and pathophysiology
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- LS3Cell Biology, Development, Stem Cells and Regeneration
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- LS5Neuroscience and Disorders of the Nervous System
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  - LS8_15Theoretical developments and modelling in environmental biology, ecology, and evolution
- LS9Biotechnology and Biosystems Engineering
  - LS9_3Bioengineering of cells, tissues, organs and organisms
  - LS9_2Applied genetics, gene editing and transgenic organisms
  - LS9_4Microbial biotechnology and bioengineering
  - LS9_5Food biotechnology and bioengineering
  - LS9_6Marine biotechnology and bioengineering
  - LS9_7Environmental biotechnology and bioengineering
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SHSocial Sciences and Humanities
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- SH3The Social World and Its Interactions
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