6533b837fe1ef96bd12a2a4c
RESEARCH PRODUCT
The nucleotide sequence of a recombinant tomato yellow leaf curl virus strain frequently detected in Sicily isolated from tomato plants carrying the Ty-1 resistance gene
A. CarusoSalvatore DavinoSalvatore DavinoStefano Pannosubject
0106 biological sciences0301 basic medicineGenes ViralSequence analysisvirusesBiology01 natural sciencesViruslaw.invention03 medical and health sciencesSolanum lycopersicumlawVirologyPlant ImmunityCultivarTomato yellow leaf curl virusGeneSicilyDisease ResistancePlant DiseasesRecombination GeneticBase SequencefungiNucleic acid sequencefood and beveragesHigh-Throughput Nucleotide SequencingSettore AGR/12 - Patologia VegetaleGeneral MedicineSequence Analysis DNAbiology.organism_classificationPlants Genetically ModifiedVirologyPlant LeavesHorticulture030104 developmental biologyBegomovirusRecombinant DNALeaf curlDisease Susceptibility010606 plant biology & botanyGeminivirus molecular evolution recombinationdescription
In July 2016, an aggressive syndrome of tomato yellow leaf curl disease was reported in Sicily in tomato plants carrying the Ty-1 resistance gene. A total of 34 samples were collected and analyzed. Twenty-seven out of the 34 samples analyzed appeared to contain only recombinant molecules. One full sequence was obtained after cloning. Alignments and plot similarity analysis showed that the genome of the recombinant, named TYLCV-IL[IT:Sic23:16], was mostly derived from tomato yellow leaf curl virus (TYLCV), with a small region of 132 nucleotides in the non-coding region between the stem-loop and the start of the V2 ORF replaced by 124 nucleotides derived from a virus of a different species, tomato yellow leaf curl Sardinia virus. All plants in which the new recombinant was detected belonged to resistant tomato cultivars.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-09-18 |