Kolorektālā audzēja šūnu līniju SW480 un SW620 ekstracelulāro vezikulu endocitozes ceļa noskaidrošana monocītu šūnu līnijā THP-1

6533b837fe1ef96bd12a2c37

RESEARCH PRODUCT

Kolorektālā audzēja šūnu līniju SW480 un SW620 ekstracelulāro vezikulu endocitozes ceļa noskaidrošana monocītu šūnu līnijā THP-1

subject

plūsmas citometrija ekstracelulārās vezikulas endocitoze Bioloģija ļaundabīgie audzēji monocīti

description

Vēža šūnu izdalītās ekstracelulārās vezikulas tiek uzskatītas par vienu no audzēju mikrovides starpšūnu komunikācijas līdzekļiem, kā arī metastātiskās nišas veidošanās iniciatoriem. Makrofāgi ir būtiska audzēja mikrovides sastāvdaļa, tāpēc darba mērķis bija noteikt audzēja šūnu izdalīto ekstracelulāro vezikulu endocitozes ceļu monocītu šūnu līnijā THP-1. Kolorektālā audzēja šūnu līniju SW480 un SW620 normoksijas un hipoksijas apstākļos izdalītās ekstracelulārās vezikulas iezīmēja ar fluorescento krāsvielu Syto RNASelect un noteica to uzņemšana THP-1 šūnās ar plūsmas citometrijas metodi. Izmantojot klatrīna, kaveolīna/lipīdu mikrodomēnu, fagocitozes un makropinocitozes inhibitorus, normoksijas apstākļos izdalītajām SW480 un SW620 EV noteica endocitozes ceļu THP-1 šūnās. Rezultātus aprēķināja datu apstrādes programmā ExpresPro un noteica endocitozes inhibitoru efektu uz iezīmēto EV uzņemšanu THP-1 šūnās. Galvenais endocitozes ceļš monocītu šūnu līnijā THP-1 ir kaveolīna atkarīgā endocitoze.

year	journal	country	edition	language
2016-01-01

https://dspace.lu.lv/dspace/handle/7/32176

PEPhysical Sciences and Engineering
- PE2Fundamental Constituents of Matter
  - PE2_1Theory of fundamental interactions
  - PE2_2 Phenomenology of fundamental interactions
  - PE2_3Experimental particle physics with accelerators
  - PE2_4Experimental particle physics without accelerators
  - PE2_6Nuclear, hadron and heavy ion physics
  - PE2_8Gas and plasma physics
  - PE2_5Classical and quantum physics of gravitational interactions
  - PE2_7Nuclear and particle astrophysics
  - PE2_9 Electromagnetism
  - PE2_10Atomic, molecular physics
  - PE2_11Ultra-cold atoms and molecules
  - PE2_18Equilibrium and non-equilibrium statistical mechanics: steady states and dynamics
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  - PE2_17Metrology and measurement
  - PE2_16Non-linear physics
  - PE2_12Optics, non-linear optics and nano-optics
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- PE1Mathematics
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  - PE1_11Theoretical aspects of partial differential equations
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  - PE1_14Mathematical statistics
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  - PE1_19Scientific computing and data processing
  - PE1_2Algebra
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  - PE1_3Number theory
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  - PE3_1Structure of solids, material growth and characterisation
  - PE3_2Mechanical and acoustical properties of condensed matter, lattice dynamics
  - PE3_3Transport properties of condensed matter
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  - PE11_10Soft materials engineering, e.g. gels, foams, colloids
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  - PE11_12Semi-conducting and magnetic materials engineering
  - PE11_13Metamaterials engineering
  - PE11_14Computational methods for materials engineering
LSLife Sciences
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  - LS1_5Lipid biology
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  - LS1_12Protein design
  - LS1_13Early translational research and drug design
  - LS1_14Innovative methods and modelling in molecular, structural and synthetic biology
  - LS1_10Synthetic biology
  - LS1_8Structural biology
  - LS1_9Molecular mechanisms of signalling processes
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  - LS2_9Metabolomics
  - LS2_10Glycomics/Lipidomics
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- LS4Physiology in Health, Disease and Ageing
  - LS4_1Organ and tissue physiology and pathophysiology
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  - LS4_2Comparative physiology
  - LS4_5Non-hormonal mechanisms of inter-organ and tissue communication
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  - LS4_11Haematopoiesis and blood diseases
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  - LS4_13Other non-communicable diseases (except disorders of the nervous system and immunity-related diseases)
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  - LS3_4Cell junctions, cell adhesion, the extracellular matrix, cell communication
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  - LS3_7Mechanobiology of cells, tissues and organs
  - LS3_8Embryogenesis, pattern formation, morphogenesis
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  - LS3_14Regeneration
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  - LS3_17Theoretical modelling in cellular, developmental and regenerative biology
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  - LS3_1Cell cycle, cell division and growth
  - LS3_2Cell senescence, cell death, autophagy, cell ageing
- LS5Neuroscience and Disorders of the Nervous System
  - LS5_1Neuronal cells
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  - LS5_5Neural networks and plasticity
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  - LS5_16Systems and computational neuroscience (e.g. modelling, simulation, brain oscillations, connectomics)
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SHSocial Sciences and Humanities
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