6533b838fe1ef96bd12a3a8c

RESEARCH PRODUCT

Application of FTIR Spectroscopy to Analyze RNA Structure

Frédéric GeinguenaudValeria MilitelloVéronique ArluisonVéronique Arluison

subject

0303 health sciencesChemistryBase pairRNAInfrared spectroscopyNucleic acid secondary structure03 medical and health scienceschemistry.chemical_compound0302 clinical medicineBiophysicsNucleic acid structureFourier transform infrared spectroscopySpectroscopy030217 neurology & neurosurgeryDNA030304 developmental biology

description

Fourier transform infrared (FTIR) spectroscopy has been widely used for the analysis of both protein and nucleic acid secondary structure. This is one of the vibration spectroscopy methods that are extremely sensitive to any change in molecular structure. While numerous reports describe how to proceed to analyze protein and deoxyribonucleic acid (DNA) structures using FTIR, reports related to the analyses of ribonucleic acids (RNAs) are few. Nevertheless, RNAs are versatile molecules involved in a multitude of roles in the cell. In this chapter, we present applications of FTIR for the structural analysis of RNA, including the analysis of helical parameters and noncanonical base pairing, often found in RNA. The effect of temperature pretreatment, which has a great impact on RNA folding, will also be discussed.

yearjournalcountryeditionlanguage
2020-01-01
https://doi.org/10.1007/978-1-0716-0278-2_10